a tubulin Search Results


α tubulin  (Proteintech)
96
Proteintech α tubulin
α Tubulin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+tubulin/pm41787480-121-47-48?v=Proteintech
Average 96 stars, based on 1 article reviews
α tubulin - by Bioz Stars, 2026-08
96/100 stars
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acetylated alpha tubulin acet tub  (Proteintech)
95
Proteintech acetylated alpha tubulin acet tub
FIGURE 2 Renal epithelial cells (URECs) derived from control 1 and CED patient with WDR35 variants presenting a typical cobblestone epithelial cell layer when grown in 2D culture (A). Human URECs following IF imaging using anti-ARL13B (green) for the ciliary membrane, <t>anti-acetylated-tubulin</t> (red) for the ciliary axoneme, PCNT (pink) to mark the base of the cilium, and DAPI (blue) for the nuclei are shown. P, CED patient; C1, control 1; C2, control 2; and C3, control 3 (B). The IF experiments revealed no significant differences in ciliogenesis between the CED patient and the combined controls (C). There was, however, a statistical difference (p < 0.01) between the CED patient and control 1, a sex- and age-matched control (D).
Acetylated Alpha Tubulin Acet Tub, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+tubulin/pm38161384-75-24-41?v=Proteintech
Average 95 stars, based on 1 article reviews
acetylated alpha tubulin acet tub - by Bioz Stars, 2026-08
95/100 stars
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coralite594 conjugated alpha tubulin monoclonal antibody  (Proteintech)
93
Proteintech coralite594 conjugated alpha tubulin monoclonal antibody
FIGURE 2 Renal epithelial cells (URECs) derived from control 1 and CED patient with WDR35 variants presenting a typical cobblestone epithelial cell layer when grown in 2D culture (A). Human URECs following IF imaging using anti-ARL13B (green) for the ciliary membrane, <t>anti-acetylated-tubulin</t> (red) for the ciliary axoneme, PCNT (pink) to mark the base of the cilium, and DAPI (blue) for the nuclei are shown. P, CED patient; C1, control 1; C2, control 2; and C3, control 3 (B). The IF experiments revealed no significant differences in ciliogenesis between the CED patient and the combined controls (C). There was, however, a statistical difference (p < 0.01) between the CED patient and control 1, a sex- and age-matched control (D).
Coralite594 Conjugated Alpha Tubulin Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+tubulin/pmc09219699-85-8-15?v=Proteintech
Average 93 stars, based on 1 article reviews
coralite594 conjugated alpha tubulin monoclonal antibody - by Bioz Stars, 2026-08
93/100 stars
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anti α tubulin  (Proteintech)
92
Proteintech anti α tubulin
FIGURE 2 Renal epithelial cells (URECs) derived from control 1 and CED patient with WDR35 variants presenting a typical cobblestone epithelial cell layer when grown in 2D culture (A). Human URECs following IF imaging using anti-ARL13B (green) for the ciliary membrane, <t>anti-acetylated-tubulin</t> (red) for the ciliary axoneme, PCNT (pink) to mark the base of the cilium, and DAPI (blue) for the nuclei are shown. P, CED patient; C1, control 1; C2, control 2; and C3, control 3 (B). The IF experiments revealed no significant differences in ciliogenesis between the CED patient and the combined controls (C). There was, however, a statistical difference (p < 0.01) between the CED patient and control 1, a sex- and age-matched control (D).
Anti α Tubulin, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+tubulin/pmc11018839-255-37-38?v=Proteintech
Average 92 stars, based on 1 article reviews
anti α tubulin - by Bioz Stars, 2026-08
92/100 stars
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a tubulin  (Proteintech)
96
Proteintech a tubulin
FIGURE 2 Renal epithelial cells (URECs) derived from control 1 and CED patient with WDR35 variants presenting a typical cobblestone epithelial cell layer when grown in 2D culture (A). Human URECs following IF imaging using anti-ARL13B (green) for the ciliary membrane, <t>anti-acetylated-tubulin</t> (red) for the ciliary axoneme, PCNT (pink) to mark the base of the cilium, and DAPI (blue) for the nuclei are shown. P, CED patient; C1, control 1; C2, control 2; and C3, control 3 (B). The IF experiments revealed no significant differences in ciliogenesis between the CED patient and the combined controls (C). There was, however, a statistical difference (p < 0.01) between the CED patient and control 1, a sex- and age-matched control (D).
A Tubulin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+tubulin/pm36702079-70-33-37?v=Proteintech
Average 96 stars, based on 1 article reviews
a tubulin - by Bioz Stars, 2026-08
96/100 stars
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pultra bcr abl wt  (Addgene inc)
91
Addgene inc pultra bcr abl wt
FIGURE 2 Renal epithelial cells (URECs) derived from control 1 and CED patient with WDR35 variants presenting a typical cobblestone epithelial cell layer when grown in 2D culture (A). Human URECs following IF imaging using anti-ARL13B (green) for the ciliary membrane, <t>anti-acetylated-tubulin</t> (red) for the ciliary axoneme, PCNT (pink) to mark the base of the cilium, and DAPI (blue) for the nuclei are shown. P, CED patient; C1, control 1; C2, control 2; and C3, control 3 (B). The IF experiments revealed no significant differences in ciliogenesis between the CED patient and the combined controls (C). There was, however, a statistical difference (p < 0.01) between the CED patient and control 1, a sex- and age-matched control (D).
Pultra Bcr Abl Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+tubulin/pmc12311789-54-18-21?v=Addgene+inc
Average 91 stars, based on 1 article reviews
pultra bcr abl wt - by Bioz Stars, 2026-08
91/100 stars
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tubulin ires puro2b  (Addgene inc)
93
Addgene inc tubulin ires puro2b
FIGURE 2 Renal epithelial cells (URECs) derived from control 1 and CED patient with WDR35 variants presenting a typical cobblestone epithelial cell layer when grown in 2D culture (A). Human URECs following IF imaging using anti-ARL13B (green) for the ciliary membrane, <t>anti-acetylated-tubulin</t> (red) for the ciliary axoneme, PCNT (pink) to mark the base of the cilium, and DAPI (blue) for the nuclei are shown. P, CED patient; C1, control 1; C2, control 2; and C3, control 3 (B). The IF experiments revealed no significant differences in ciliogenesis between the CED patient and the combined controls (C). There was, however, a statistical difference (p < 0.01) between the CED patient and control 1, a sex- and age-matched control (D).
Tubulin Ires Puro2b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+tubulin/ppr0500845-306-35-38?v=Addgene+inc
Average 93 stars, based on 1 article reviews
tubulin ires puro2b - by Bioz Stars, 2026-08
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ppamcherry a tubulin plasmid  (Addgene inc)
93
Addgene inc ppamcherry a tubulin plasmid
FIGURE 2 Renal epithelial cells (URECs) derived from control 1 and CED patient with WDR35 variants presenting a typical cobblestone epithelial cell layer when grown in 2D culture (A). Human URECs following IF imaging using anti-ARL13B (green) for the ciliary membrane, <t>anti-acetylated-tubulin</t> (red) for the ciliary axoneme, PCNT (pink) to mark the base of the cilium, and DAPI (blue) for the nuclei are shown. P, CED patient; C1, control 1; C2, control 2; and C3, control 3 (B). The IF experiments revealed no significant differences in ciliogenesis between the CED patient and the combined controls (C). There was, however, a statistical difference (p < 0.01) between the CED patient and control 1, a sex- and age-matched control (D).
Ppamcherry A Tubulin Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+tubulin/pmc09406521-83-23-25?v=Addgene+inc
Average 93 stars, based on 1 article reviews
ppamcherry a tubulin plasmid - by Bioz Stars, 2026-08
93/100 stars
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light emitting calcium indicator jgcamp8m  (Addgene inc)
85
Addgene inc light emitting calcium indicator jgcamp8m
FIGURE 2 Renal epithelial cells (URECs) derived from control 1 and CED patient with WDR35 variants presenting a typical cobblestone epithelial cell layer when grown in 2D culture (A). Human URECs following IF imaging using anti-ARL13B (green) for the ciliary membrane, <t>anti-acetylated-tubulin</t> (red) for the ciliary axoneme, PCNT (pink) to mark the base of the cilium, and DAPI (blue) for the nuclei are shown. P, CED patient; C1, control 1; C2, control 2; and C3, control 3 (B). The IF experiments revealed no significant differences in ciliogenesis between the CED patient and the combined controls (C). There was, however, a statistical difference (p < 0.01) between the CED patient and control 1, a sex- and age-matched control (D).
Light Emitting Calcium Indicator Jgcamp8m, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+tubulin/pm40623068-315-21-28?v=Addgene+inc
Average 85 stars, based on 1 article reviews
light emitting calcium indicator jgcamp8m - by Bioz Stars, 2026-08
85/100 stars
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immunofluorescence  (Biorbyt)
93
Biorbyt immunofluorescence
FIGURE 2 Renal epithelial cells (URECs) derived from control 1 and CED patient with WDR35 variants presenting a typical cobblestone epithelial cell layer when grown in 2D culture (A). Human URECs following IF imaging using anti-ARL13B (green) for the ciliary membrane, <t>anti-acetylated-tubulin</t> (red) for the ciliary axoneme, PCNT (pink) to mark the base of the cilium, and DAPI (blue) for the nuclei are shown. P, CED patient; C1, control 1; C2, control 2; and C3, control 3 (B). The IF experiments revealed no significant differences in ciliogenesis between the CED patient and the combined controls (C). There was, however, a statistical difference (p < 0.01) between the CED patient and control 1, a sex- and age-matched control (D).
Immunofluorescence, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+tubulin/pm41052774-90-7-14?v=Biorbyt
Average 93 stars, based on 1 article reviews
immunofluorescence - by Bioz Stars, 2026-08
93/100 stars
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constructs pmegfp a tubulin c1  (Addgene inc)
92
Addgene inc constructs pmegfp a tubulin c1
FIGURE 2 Renal epithelial cells (URECs) derived from control 1 and CED patient with WDR35 variants presenting a typical cobblestone epithelial cell layer when grown in 2D culture (A). Human URECs following IF imaging using anti-ARL13B (green) for the ciliary membrane, <t>anti-acetylated-tubulin</t> (red) for the ciliary axoneme, PCNT (pink) to mark the base of the cilium, and DAPI (blue) for the nuclei are shown. P, CED patient; C1, control 1; C2, control 2; and C3, control 3 (B). The IF experiments revealed no significant differences in ciliogenesis between the CED patient and the combined controls (C). There was, however, a statistical difference (p < 0.01) between the CED patient and control 1, a sex- and age-matched control (D).
Constructs Pmegfp A Tubulin C1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+tubulin/pmc05638588-443-1-12?v=Addgene+inc
Average 92 stars, based on 1 article reviews
constructs pmegfp a tubulin c1 - by Bioz Stars, 2026-08
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antibody against tubb2a  (Novus Biologicals)
94
Novus Biologicals antibody against tubb2a
Overall workflow and results of key gene screening and survival analysis. (A) Volcano plot of differentially expressed genes in GSE166163 . (B) Venn diagram showing the intersection between function-related genes from GeneCards (cholesterol metabolism, cell proliferation, and apoptosis) and upregulated genes (n = 139). (C) Cross-validation plot of the LASSO regression model used to determine the optimal λ value and select feature genes. (D) Variable importance ranking of candidate genes generated using the random forest model. (E) Twenty-three core genes obtained from the intersection of LASSO and random forest results. (F) Univariate Cox regression analysis based on TCGA-LIHC identifying nine genes significantly associated with overall survival (OS). (G–L) Kaplan-Meier survival curves of six representative genes (LOX, MMP3, MMP9, TFE3, <t>TUBB2A,</t> and UCHL1).
Antibody Against Tubb2a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+tubulin/pmc13033567-118-2-5?v=Novus+Biologicals
Average 94 stars, based on 1 article reviews
antibody against tubb2a - by Bioz Stars, 2026-08
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Image Search Results


FIGURE 2 Renal epithelial cells (URECs) derived from control 1 and CED patient with WDR35 variants presenting a typical cobblestone epithelial cell layer when grown in 2D culture (A). Human URECs following IF imaging using anti-ARL13B (green) for the ciliary membrane, anti-acetylated-tubulin (red) for the ciliary axoneme, PCNT (pink) to mark the base of the cilium, and DAPI (blue) for the nuclei are shown. P, CED patient; C1, control 1; C2, control 2; and C3, control 3 (B). The IF experiments revealed no significant differences in ciliogenesis between the CED patient and the combined controls (C). There was, however, a statistical difference (p < 0.01) between the CED patient and control 1, a sex- and age-matched control (D).

Journal: Frontiers in molecular biosciences

Article Title: Ciliary phenotyping in renal epithelial cells in a cranioectodermal dysplasia patient with WDR35 variants.

doi: 10.3389/fmolb.2023.1285790

Figure Lengend Snippet: FIGURE 2 Renal epithelial cells (URECs) derived from control 1 and CED patient with WDR35 variants presenting a typical cobblestone epithelial cell layer when grown in 2D culture (A). Human URECs following IF imaging using anti-ARL13B (green) for the ciliary membrane, anti-acetylated-tubulin (red) for the ciliary axoneme, PCNT (pink) to mark the base of the cilium, and DAPI (blue) for the nuclei are shown. P, CED patient; C1, control 1; C2, control 2; and C3, control 3 (B). The IF experiments revealed no significant differences in ciliogenesis between the CED patient and the combined controls (C). There was, however, a statistical difference (p < 0.01) between the CED patient and control 1, a sex- and age-matched control (D).

Article Snippet: After fixation with 2% PFA (Sigma), permeabilization with 1% Triton, and blocking with 2% BSA (Sigma), the cells were incubated with primary antibodies targeting acetylated-alpha-tubulin (Acet-Tub) at 1:1000 (mouse monoclonal T6793, Sigma-Aldrich), ADP-ribosylation factor-like protein 13B (ARL13B) at 1:500 (rabbit polyclonal, Proteintech Group), and pericentrin (PCNT) at 1:1000 (mouse monoclonal, Abcam Cambridge), followed by incubation with fluor-labeled secondary antibodies: anti-mouse Alexa Fluor 568, anti-rabbit Alexa Fluor 488, and anti-mouse Alexa Fluor 647 from Thermo Fisher Scientific.

Techniques: Derivative Assay, Control, Imaging, Membrane

FIGURE 3 Cilium length, height, and width were analyzed using two antibodies specific for Acet-Tub and ARL13B. Examination of the cilia width (A axis) revealed highly significant differences between the CED patient and controls for both markers (Acet-Tub and ARL13B: p < 0.0001) (A, B). Evaluation of the ciliary height showed no differences between the CED patient and controls (C, D). Analysis of the ciliary length revealed longer cilia in the CED patient as compared to controls for both proteins (Acet-Tub: p < 0.01 and ARL13B: p < 0.05) (E, F).

Journal: Frontiers in molecular biosciences

Article Title: Ciliary phenotyping in renal epithelial cells in a cranioectodermal dysplasia patient with WDR35 variants.

doi: 10.3389/fmolb.2023.1285790

Figure Lengend Snippet: FIGURE 3 Cilium length, height, and width were analyzed using two antibodies specific for Acet-Tub and ARL13B. Examination of the cilia width (A axis) revealed highly significant differences between the CED patient and controls for both markers (Acet-Tub and ARL13B: p < 0.0001) (A, B). Evaluation of the ciliary height showed no differences between the CED patient and controls (C, D). Analysis of the ciliary length revealed longer cilia in the CED patient as compared to controls for both proteins (Acet-Tub: p < 0.01 and ARL13B: p < 0.05) (E, F).

Article Snippet: After fixation with 2% PFA (Sigma), permeabilization with 1% Triton, and blocking with 2% BSA (Sigma), the cells were incubated with primary antibodies targeting acetylated-alpha-tubulin (Acet-Tub) at 1:1000 (mouse monoclonal T6793, Sigma-Aldrich), ADP-ribosylation factor-like protein 13B (ARL13B) at 1:500 (rabbit polyclonal, Proteintech Group), and pericentrin (PCNT) at 1:1000 (mouse monoclonal, Abcam Cambridge), followed by incubation with fluor-labeled secondary antibodies: anti-mouse Alexa Fluor 568, anti-rabbit Alexa Fluor 488, and anti-mouse Alexa Fluor 647 from Thermo Fisher Scientific.

Techniques:

FIGURE 4 Volume measurements of ARL13B, Acet-Tub, and PCNT. The amount of all markers was calculated and revealed a highly significant increase in volume for all three proteins (Acet-Tub, ARL13B, and PCNT: p < 0.0001) (A–C).

Journal: Frontiers in molecular biosciences

Article Title: Ciliary phenotyping in renal epithelial cells in a cranioectodermal dysplasia patient with WDR35 variants.

doi: 10.3389/fmolb.2023.1285790

Figure Lengend Snippet: FIGURE 4 Volume measurements of ARL13B, Acet-Tub, and PCNT. The amount of all markers was calculated and revealed a highly significant increase in volume for all three proteins (Acet-Tub, ARL13B, and PCNT: p < 0.0001) (A–C).

Article Snippet: After fixation with 2% PFA (Sigma), permeabilization with 1% Triton, and blocking with 2% BSA (Sigma), the cells were incubated with primary antibodies targeting acetylated-alpha-tubulin (Acet-Tub) at 1:1000 (mouse monoclonal T6793, Sigma-Aldrich), ADP-ribosylation factor-like protein 13B (ARL13B) at 1:500 (rabbit polyclonal, Proteintech Group), and pericentrin (PCNT) at 1:1000 (mouse monoclonal, Abcam Cambridge), followed by incubation with fluor-labeled secondary antibodies: anti-mouse Alexa Fluor 568, anti-rabbit Alexa Fluor 488, and anti-mouse Alexa Fluor 647 from Thermo Fisher Scientific.

Techniques:

Overall workflow and results of key gene screening and survival analysis. (A) Volcano plot of differentially expressed genes in GSE166163 . (B) Venn diagram showing the intersection between function-related genes from GeneCards (cholesterol metabolism, cell proliferation, and apoptosis) and upregulated genes (n = 139). (C) Cross-validation plot of the LASSO regression model used to determine the optimal λ value and select feature genes. (D) Variable importance ranking of candidate genes generated using the random forest model. (E) Twenty-three core genes obtained from the intersection of LASSO and random forest results. (F) Univariate Cox regression analysis based on TCGA-LIHC identifying nine genes significantly associated with overall survival (OS). (G–L) Kaplan-Meier survival curves of six representative genes (LOX, MMP3, MMP9, TFE3, TUBB2A, and UCHL1).

Journal: Frontiers in Molecular Biosciences

Article Title: TUBB2A expression and its prognostic significance in hepatocellular carcinoma revealed by cholesterol-metabolism-related gene profiling

doi: 10.3389/fmolb.2026.1778506

Figure Lengend Snippet: Overall workflow and results of key gene screening and survival analysis. (A) Volcano plot of differentially expressed genes in GSE166163 . (B) Venn diagram showing the intersection between function-related genes from GeneCards (cholesterol metabolism, cell proliferation, and apoptosis) and upregulated genes (n = 139). (C) Cross-validation plot of the LASSO regression model used to determine the optimal λ value and select feature genes. (D) Variable importance ranking of candidate genes generated using the random forest model. (E) Twenty-three core genes obtained from the intersection of LASSO and random forest results. (F) Univariate Cox regression analysis based on TCGA-LIHC identifying nine genes significantly associated with overall survival (OS). (G–L) Kaplan-Meier survival curves of six representative genes (LOX, MMP3, MMP9, TFE3, TUBB2A, and UCHL1).

Article Snippet: The primary antibody against TUBB2A (Novus, catalog number NBP3-16484-100 μL) was diluted following the manufacturer’s guidelines and applied to tissue sections, which were incubated 2 h. Then sections were subjected to three washes using Tris-buffered saline, with each wash performed for 5 min.

Techniques: Biomarker Discovery, Generated

Expression characteristics of key genes in the GSE151530 single-cell atlas. (A) t-SNE plot displaying single-cell clusters and annotation of major cell types. (B) Feature plots showing the distribution of candidate genes (LOX, MMP3, MMP9, TFE3, TUBB2A, and UCHL1) across different cell subpopulations. (C) Violin plots illustrating differential expression of TUBB2A between HCC and normal tissues across various cell types.

Journal: Frontiers in Molecular Biosciences

Article Title: TUBB2A expression and its prognostic significance in hepatocellular carcinoma revealed by cholesterol-metabolism-related gene profiling

doi: 10.3389/fmolb.2026.1778506

Figure Lengend Snippet: Expression characteristics of key genes in the GSE151530 single-cell atlas. (A) t-SNE plot displaying single-cell clusters and annotation of major cell types. (B) Feature plots showing the distribution of candidate genes (LOX, MMP3, MMP9, TFE3, TUBB2A, and UCHL1) across different cell subpopulations. (C) Violin plots illustrating differential expression of TUBB2A between HCC and normal tissues across various cell types.

Article Snippet: The primary antibody against TUBB2A (Novus, catalog number NBP3-16484-100 μL) was diluted following the manufacturer’s guidelines and applied to tissue sections, which were incubated 2 h. Then sections were subjected to three washes using Tris-buffered saline, with each wash performed for 5 min.

Techniques: Expressing, Single Cell, Quantitative Proteomics

Expression of TUBB2A in HCC tissues and its prognostic significance. (A) Above: HE staining showing tissue morphology; below: immunohistochemical staining showing TUBB2A expression in the cytoplasm of hepatocellular carcinoma tissues, with higher expression in tumor tissues compared to adjacent normal tissues. Left: adjacent normal tissue with low TUBB2A expression, middle: HCC with lower TUBB2A expression, right: HCC with higher TUBB2A expression (magnification ×200). (B) RT-qPCR analysis of TUBB2A expression in normal and HCC tissues. (C) Western blot bands and quantitative analysis of TUBB2A expression in normal and tumor tissues (N: normal; T: tumor). (D) Kaplan-Meier survival curves of HCC patients stratified by low versus high TUBB2A expression (n = 121). (E) Kaplan–Meier survival curves of HCC patients with tumor diameter ≥5 cm, stratified by TUBB2A expression level (n = 59). Note: *** P ≤ 0.001; **** P ≤ 0.0001.

Journal: Frontiers in Molecular Biosciences

Article Title: TUBB2A expression and its prognostic significance in hepatocellular carcinoma revealed by cholesterol-metabolism-related gene profiling

doi: 10.3389/fmolb.2026.1778506

Figure Lengend Snippet: Expression of TUBB2A in HCC tissues and its prognostic significance. (A) Above: HE staining showing tissue morphology; below: immunohistochemical staining showing TUBB2A expression in the cytoplasm of hepatocellular carcinoma tissues, with higher expression in tumor tissues compared to adjacent normal tissues. Left: adjacent normal tissue with low TUBB2A expression, middle: HCC with lower TUBB2A expression, right: HCC with higher TUBB2A expression (magnification ×200). (B) RT-qPCR analysis of TUBB2A expression in normal and HCC tissues. (C) Western blot bands and quantitative analysis of TUBB2A expression in normal and tumor tissues (N: normal; T: tumor). (D) Kaplan-Meier survival curves of HCC patients stratified by low versus high TUBB2A expression (n = 121). (E) Kaplan–Meier survival curves of HCC patients with tumor diameter ≥5 cm, stratified by TUBB2A expression level (n = 59). Note: *** P ≤ 0.001; **** P ≤ 0.0001.

Article Snippet: The primary antibody against TUBB2A (Novus, catalog number NBP3-16484-100 μL) was diluted following the manufacturer’s guidelines and applied to tissue sections, which were incubated 2 h. Then sections were subjected to three washes using Tris-buffered saline, with each wash performed for 5 min.

Techniques: Expressing, Staining, Immunohistochemical staining, Quantitative RT-PCR, Western Blot

Effects of TUBB2A knockdown on the malignant behavior of HCC cells. (A) Effects of TUBB2A knockdown on the clonogenic capacity of Hep3B cells. (B) Effects of TUBB2A knockdown on Hep3B cell viability as measured by the CCK-8 assa. (C) Effects of TUBB2A knockdown on the migratory and invasive abilities of Hep3B cells, as shown by representative images and quantitative analysis of cell numbers. (D) Representative images and quantitative analysis of scratch-wound healing in Hep3B cells following TUBB2A knockdown. Note: NC group, negative control; si group, TUBB2A knockdown. * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001.

Journal: Frontiers in Molecular Biosciences

Article Title: TUBB2A expression and its prognostic significance in hepatocellular carcinoma revealed by cholesterol-metabolism-related gene profiling

doi: 10.3389/fmolb.2026.1778506

Figure Lengend Snippet: Effects of TUBB2A knockdown on the malignant behavior of HCC cells. (A) Effects of TUBB2A knockdown on the clonogenic capacity of Hep3B cells. (B) Effects of TUBB2A knockdown on Hep3B cell viability as measured by the CCK-8 assa. (C) Effects of TUBB2A knockdown on the migratory and invasive abilities of Hep3B cells, as shown by representative images and quantitative analysis of cell numbers. (D) Representative images and quantitative analysis of scratch-wound healing in Hep3B cells following TUBB2A knockdown. Note: NC group, negative control; si group, TUBB2A knockdown. * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001.

Article Snippet: The primary antibody against TUBB2A (Novus, catalog number NBP3-16484-100 μL) was diluted following the manufacturer’s guidelines and applied to tissue sections, which were incubated 2 h. Then sections were subjected to three washes using Tris-buffered saline, with each wash performed for 5 min.

Techniques: Knockdown, CCK-8 Assay, Negative Control

Effects of TUBB2A overexpression on the malignant phenotypes of HCC cells. (A) Effects of TUBB2A overexpression on the clonogenic capacity of LM3 cells. (B) Effects of TUBB2A overexpression on LM3 cell viability as measured by the CCK-8 assay. (C) Representative images and quantification of cell numbers showing the effects of TUBB2A overexpression on migration and invasion in LM3 cells. (D) Representative images and quantitative analysis of scratch-wound healing in LM3 cells following TUBB2A overexpression. Note: NC group, negative control group; OE group, TUBB2A overexpression group. * P ≤ 0.05; ** P ≤ 0.01. *** P ≤ 0.001; **** P ≤ 0.0001.

Journal: Frontiers in Molecular Biosciences

Article Title: TUBB2A expression and its prognostic significance in hepatocellular carcinoma revealed by cholesterol-metabolism-related gene profiling

doi: 10.3389/fmolb.2026.1778506

Figure Lengend Snippet: Effects of TUBB2A overexpression on the malignant phenotypes of HCC cells. (A) Effects of TUBB2A overexpression on the clonogenic capacity of LM3 cells. (B) Effects of TUBB2A overexpression on LM3 cell viability as measured by the CCK-8 assay. (C) Representative images and quantification of cell numbers showing the effects of TUBB2A overexpression on migration and invasion in LM3 cells. (D) Representative images and quantitative analysis of scratch-wound healing in LM3 cells following TUBB2A overexpression. Note: NC group, negative control group; OE group, TUBB2A overexpression group. * P ≤ 0.05; ** P ≤ 0.01. *** P ≤ 0.001; **** P ≤ 0.0001.

Article Snippet: The primary antibody against TUBB2A (Novus, catalog number NBP3-16484-100 μL) was diluted following the manufacturer’s guidelines and applied to tissue sections, which were incubated 2 h. Then sections were subjected to three washes using Tris-buffered saline, with each wash performed for 5 min.

Techniques: Over Expression, CCK-8 Assay, Migration, Negative Control

Effects of TUBB2A on the in vivo tumorigenic ability of HCC cells. (A) Representative images of subcutaneous tumors formed in nude mice in the two groups. Above: Subcutaneous tumors of the control group; below: Subcutaneous tumors of the shTUBB2A group. (B) Growth curves showing changes in subcutaneous tumor volume in the two groups of nude mice. (C) Comparison of subcutaneous tumor weight between the two groups of nude mice. (D) Above: HE staining showing tissue morphology; below: IHC Ki-67 staining. Left: TUBB2A control group nude mouse tumor with low Ki-67 expression; right: TUBB2A knockdown group nude mouse tumor with high Ki-67 expression (magnification ×200). (E) Quantification of Ki-67 IHC score in the subcutaneous tumors. Note: *** P ≤ 0.001; **** P ≤ 0.0001.

Journal: Frontiers in Molecular Biosciences

Article Title: TUBB2A expression and its prognostic significance in hepatocellular carcinoma revealed by cholesterol-metabolism-related gene profiling

doi: 10.3389/fmolb.2026.1778506

Figure Lengend Snippet: Effects of TUBB2A on the in vivo tumorigenic ability of HCC cells. (A) Representative images of subcutaneous tumors formed in nude mice in the two groups. Above: Subcutaneous tumors of the control group; below: Subcutaneous tumors of the shTUBB2A group. (B) Growth curves showing changes in subcutaneous tumor volume in the two groups of nude mice. (C) Comparison of subcutaneous tumor weight between the two groups of nude mice. (D) Above: HE staining showing tissue morphology; below: IHC Ki-67 staining. Left: TUBB2A control group nude mouse tumor with low Ki-67 expression; right: TUBB2A knockdown group nude mouse tumor with high Ki-67 expression (magnification ×200). (E) Quantification of Ki-67 IHC score in the subcutaneous tumors. Note: *** P ≤ 0.001; **** P ≤ 0.0001.

Article Snippet: The primary antibody against TUBB2A (Novus, catalog number NBP3-16484-100 μL) was diluted following the manufacturer’s guidelines and applied to tissue sections, which were incubated 2 h. Then sections were subjected to three washes using Tris-buffered saline, with each wash performed for 5 min.

Techniques: In Vivo, Control, Comparison, Staining, Expressing, Knockdown